🔍 What is Custom mRNA Synthesis?
Custom mRNA synthesis refers to the process of designing and producing messenger RNA molecules tailored to specific research or therapeutic needs. These mRNAs can encode antigens, therapeutic proteins, or regulatory elements, making them invaluable tools in:
- Vaccine development
- Gene expression studies
- Protein replacement therapies
- Autoimmune disease modeling
- Preclinical and clinical trials
With technologies such as in vitro transcription (IVT) , capping methods , poly(A) tailing , and modified nucleotides , researchers can now produce high-quality, stable, and functional mRNA at scale.
Autoimmune IFN Mouse Model mRNA Vaccine
📌 Client :
- Prof. Lieven Gevaert , Bio Engineer & CEO
- Gentaur Genprice
🧪 Application :
- Autoimmune Disease Modeling.
- Targeting interferon (IFN) pathways using custom-designed mRNA constructs.
✅ Services Provided :
☑️ Plasmid Amplification.
☑️ mRNA Synthesis.
☑️ Lipid Nanoparticle (LNP) Encapsulation.
🔬 mRNA Specifications :
Parameter | Details |
|---|---|
RNA Size | 160 nt |
Structure | Linear |
Ionizable Lipid | ALC-0315 |
Buffer | Supplied by provider |
Final Yield | mg-scale LNP |
This case study demonstrates our capability to deliver fully integrated mRNA solutions , from plasmid amplification through LNP formulation, tailored to complex preclinical applications.
🧪 Key Features of Our Custom mRNA Services
1. Custom Sequence Design
We support multi-antigen mRNA constructs for advanced immune modulation studies. In this project, the client requested multiple antigens per mRNA strand, totaling 35 amino acids .
2. Plasmid Amplification
We amplified cyclic plasmid templates designed for post-transcriptional capping , ensuring compatibility with downstream transcription processes.
3. mRNA Synthesis
- Type : Linearized plasmid template
- Capping Method : Post-transcriptional capping
- Poly(A) Tailing : Required
- Modified Nucleotide : UTP
- Grade : Research Grade
- Buffer : Nuclease-Free Water
4. LNP Encapsulation
- Encapsulation Type : Linear RNA
- Ionizable Lipid : ALC-0315
- QC Parameters : Particle size, PDI, concentration, and encapsulation rate
5. Quality Control
Basic QC included :
- OD260/280.
- Formaldehyde gel electrophoresis.
- mRNA integrity.
Optional QC available upon request :
- Endotoxin detection.
- dsRNA analysis.
- In vivo expression verification.
📈 Why Choose Custom mRNA Services for Autoimmune Research?
Autoimmune diseases like multiple sclerosis , type 1 diabetes , and lupus involve dysregulated interferon signaling. By delivering precise mRNA constructs encoding interferon modulators , researchers can explore novel immunotherapies and model disease progression more accurately.
Using mRNA vaccines in animal models enables :
- Targeted protein expression.
- Controlled immune activation.
- Repeatable and scalable results.
- Rapid prototyping of antigenic variants.
Our platform allows for seamless transition from discovery to preclinical testing , supporting both academic and industrial clients globally.
🧫 Applications of Custom mRNA Service
Application | Description |
|---|---|
Vaccine Development | Infectious diseases, cancer vaccines, autoimmune therapies |
Gene Therapy | Protein replacement, gene editing (e.g., CRISPR) |
Immune Modulation Studies | Cytokine expression, checkpoint molecule delivery |
Functional Genomics | Overexpression, knockdown, reporter assays |
Preclinical Models | Mouse, rat, and non-human primate studies |
🧬 Supported Technologies
✅ Modified Nucleotides:
- UTP
- pUTP
- N1-Me-pUTP
✅ Capping Methods :
- Co-transcriptional capping (Cap1)
- Post-transcriptional capping
✅ Poly(A) Tail Options:
- Standard poly(A) tailing
- Defined-length tails for enhanced stability
✅ Delivery Systems:
- Lipid nanoparticles (LNPs) using ionizable lipids:
- ALC-0315
- SM-102
📊 Quality Assurance & Validation
We ensure that all synthesized mRNAs meet rigorous quality standards before delivery:
Basic QC :
- Spectrophotometric Analysis (OD260/280)
- Formaldehyde Gel Electrophoresis
- Cap Integrity & mRNA Length Verification
Optional Advanced QC :
- dsRNA Detection
- Endotoxin Testing
- Residual DNA/RNA/Protein Detection
- Expression Validation (Western blot, ELISA, Fluorescence Imaging)
🧾 Real-World Collaboration : Gentaur Genprice
This collaboration with Prof. Lieven Gevaert’s team highlights our ability to provide end-to-end support for complex mRNA projects involving autoimmune targets.
By leveraging our expertise in :
- mRNA design and synthesis
- plasmid preparation
- LNP formulation
- quality control and validation
We help clients accelerate their research timelines and reduce development costs.
💡 Looking Ahead: Future of mRNA Therapeutics
The global demand for mRNA-based therapies is growing rapidly, driven by breakthroughs in :
- mRNA vaccines
- personalized cancer vaccines
- protein replacement therapies
- CRISPR delivery systems
At Gentaur, we remain committed to staying ahead of these advancements by continuously improving our mRNA production capabilities , expanding our delivery platforms , and offering tailored solutions to meet evolving scientific needs.
📩 Ready to Start Your Custom mRNA Project?
Whether you're developing a novel mRNA vaccine , exploring gene regulation , or building animal models for autoimmune diseases , Gentaur provides the tools, expertise, and flexibility to bring your ideas to life.
📧 Contact us today to discuss your custom mRNA requirements and receive a personalized quote.
📞 +32 16 89 55 44
📍 Voortstraat 49, 1910 Kampenhout, Belgium
Based on the information provided in the Gentaur mRNA custom service request form , Gentaur Genprice, led by Bio Engineer & CEO Lieven Gevaert , is actively engaged in developing a custom mRNA vaccine targeting autoimmune interferon (IFN) pathways in a mouse model . The project involves a comprehensive workflow including plasmid amplification, mRNA synthesis , and LNP encapsulation using ALC-0315 ionizable lipid for delivery. The synthesized mRNA is linear, with a length of 160 nucleotides , produced using post-transcriptional capping , UTP modification , and supplied in nuclease-free water . Basic quality control parameters such as OD260/280 ratio, mRNA integrity, formaldehyde gel electrophoresis, LNP particle size, PDI, and encapsulation efficiency are included to ensure high-quality output tailored for preclinical research in autoimmune disease modeling.